Journal: Translational Cancer Research

Article Title: The suppressing effects of VEGF-mediated angiogenesis at different administration sequences of apatinib and transarterial embolization in vivo

doi: 10.21037/tcr.2019.12.97

Figure Lengend Snippet: Immunohistochemical staining showed a positive CD34 reaction (×200). Positive CD34 was identified by dark-brown staining. (A) Control group (CG); (B) combined Group 1 (CG1); (C) combined Group 2 (CG2); (D) combined Group 3 (CG3).

Article Snippet: Both rabbit anti-rat VEGF-165 polyclonal antibody and rabbit anti-rat CD34 polyclonal antibody were purchased from BOSTER, Inc, Wuhan, China.

Techniques: Immunohistochemical staining, Staining, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Involvement of stromal cell-derived factor-1α (SDF-1α), stem cell factor (SCF), fractalkine (FKN) and VEGF in TSG protection against intimal hyperplasia in rat balloon injury.

doi: 10.1016/j.biopha.2018.12.030

Figure Lengend Snippet: Fig. 5. TSG increased the protein expression of VEGF in serum and the protein expression of CD34 in vessel wall after balloon injury. A: The representative sections of arteries with immunostaining for CD34 (10 ×). B: The representative sections of arteries with immunostaining for CD34 (40 ×). C: Analysis for protein expression of VEGF in serum. n = 3-5. *P < 0.05 vs Sham group, #P < 0.05 vs Model group.

Article Snippet: CD34 goat anti-rat polyclonal antibody was purchased from R&D System (USA).

Techniques: Expressing, Immunostaining

Journal: PLoS ONE

Article Title: Therapeutic Effects of Human Amniotic Fluid-Derived Stem Cells on Renal Interstitial Fibrosis in a Murine Model of Unilateral Ureteral Obstruction

doi: 10.1371/journal.pone.0065042

Figure Lengend Snippet: ( A ) Morphology of hAFSCs under high magnification. After 3 passages in culture, the cells were spindle-shaped with relatively large nuclei; conjugate nuclei were also present (bar, 100 µm). ( B ) Morphology of hAFSCs after 2 days of culture. The cells exhibited a needle-like shaped appearance and grew with no visible directionality (bar, 50 µm). ( C ) Morphology of hAFSCs after 10 days of culture. Cells grow as a vortex and a crater (bar, 50 µm). ( D ) The third passage hAFSCs stained with Oct-4. Cells stained positive for Oct-4, a stem cell surface marker (bar, 50 µm). ( E ) CM-DiI-labeled hAFSCs. ( F ) Double immunofluorescent staining of a kidney section taken from an animal injected with hAFSCs at day 14 post-injection. Positive HLA-class I staining confirms the presence of hAFSCs at day 14 post-transplantation in the frozen renal tissue sections (bar, 50 µm).

Article Snippet: To perform immunohistochemical staining, rabbit anti-hypoxia inducible factor-1α (HIF-1α) polyclonal antibodies, rabbit anti-CD34 polyclonal antibodies (1∶50) (Boster Biotechnology), rabbit anti-TGF-β1 polyclonal antibodies (1∶50) (Santa Cruz Biotechnology), rabbit anti–MCP-1 (1∶100, Boster Biotechnology), rabbit anti-E-cadherin protein (1∶100, Zhongshan, China), rabbit anti-Collagen-I (1∶100, KeyGEN Bio TECH, China), and rabbit anti–Ki67 (1∶50, Boster Biotechnology) were used for immunohistochemical procedures.

Techniques: Staining, Marker, Labeling, Injection, Transplantation Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats

doi: 10.3892/etm.2015.2592

Figure Lengend Snippet: Number of white blood cells in the peripheral blood (x10 9 /l) and the ratio of CD34 + cells among the mononuclear cells (%).

Article Snippet: Rabbit anti-rat CD34 + polyclonal antibody (dilution, 1:200; cat. no. bs-0754R; Beijing Bioss Biosynthesis Biotechnology Co., Ltd.) was used as the primary antibody for overnight incubation at 4°C.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats

doi: 10.3892/etm.2015.2592

Figure Lengend Snippet: Number of cluster of differentiation 34+ cells in four groups of rat renal tissues (arrows indicate immunohistochemical staining; magnification, x200). (A–C) The (A) normal control, (B) treatment control and (C) model groups at day 5; (D–F) the model group at (D) day 10, (E) day 17 and (F) day 24; (G–J) the treatment group at (G) day 5, (H) day 10, (I) day 17 and (J) day 24.

Article Snippet: Rabbit anti-rat CD34 + polyclonal antibody (dilution, 1:200; cat. no. bs-0754R; Beijing Bioss Biosynthesis Biotechnology Co., Ltd.) was used as the primary antibody for overnight incubation at 4°C.

Techniques: Immunohistochemical staining, Staining

Journal: Cancer Medicine

Article Title: Therapeutic potential of targeting protein for Xklp2 silencing for pancreatic cancer

doi: 10.1002/cam4.453

Figure Lengend Snippet: Effects of TPX2 silencing on the expression of angiogenesis-associated factors in a xenograft mouse model. (A) Profiling of angiogenesis and antiangiogenesis factors was performed using Human Anginogenesis Arrays in tumors treated with Control siRNA- or TPX2 siRNA-2 in a xenograft mouse model ( n = 3 in each group). The expression of IGFBP-3 (circled) was identified in tumors treated with TPX2 siRNA and Control siRNA using the angiogenesis arrays (left). The IGFBP-3 expression levels were shown relative to the levels in tumors treated with Control siRNA (right). * P < 0.05 vs. Control siRNA-treated tumor. (B) The expression of IGFBP-3 and CD34 was analyzed by immunohistochemistry in xenograft tumors treated with either PBS (left), Control siRNA (middle), or TPX2 siRNA (right). The graphs show the proportion of IGFBP-3-positive cells relative to the total number of cells (left) and the number of CD34-positive cells (right) in 10 randomly selected high- power fields per section. * P < 0.05 vs. PBS-treated tumor. ** P < 0.05 vs. Control siRNA-treated tumor. TPX2, targeting protein for Xklp2; PBS, phosphate-buffered saline; IGFBP3, insulin-like growth factor-binding protein-3.

Article Snippet: CD34 and Ki67 were stained automatically using rabbit anti-CD34 polyclonal antibody (Bioss, Woburn, MA) and rabbit anti-Ki67 monoclonal antibodies (Ventana Medical Systems, Tucson, AZ).

Techniques: Expressing, Immunohistochemistry, Binding Assay